› 4dz1

X-ray diffraction
1.9Å resolution

Crystal structure of DalS, an ATP binding cassette transporter for D-alanine from Salmonella enterica

Released:
DOI: 10.2210/pdb4dz1/pdb
PDB entry 4dz1 coloured by chain, front view.
PDB entry 4dz1 coloured by chain, side view.
PDB entry 4dz1 coloured by chain, top view.
Source organism: Salmonella enterica
Primary publication:
Characterization of DalS, an ATP-binding cassette transporter for D-alanine, and its role in pathogenesis in Salmonella enterica.
Osborne SE, Tuinema BR, Mok MC, Lau PS, Bui NK, Tomljenovic-Berube AM, Vollmer W, Zhang K, Junop M, Coombes BK
J Biol Chem 287 15242-50 (2012)
PMID: 22418438

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DalS D-Alanine transporter Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 29.77 KDa
Source organism: Salmonella enterica
Expression system: Escherichia coli
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand DAL 1 x DAL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6122
Unit cell:
a: 81.076Å b: 81.076Å c: 187.926Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.19 0.203
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Selective substrate uptake: The role of ATP-binding cassette (ABC) importers in pathogenesis.
Tanaka et al. (2018)
1 more

1 mention without citation

Characterization of DalS, an ATP-binding cassette transporter for D-alanine, and its role in pathogenesis in Salmonella enterica.
Osborne et al. (2012)