4dxn

X-ray diffraction
1.85Å resolution

Crystal Structure of a reconstructed Kaede-type Red Fluorescent Protein, Least Evolved Ancestor (LEA)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-164403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LEAST EVOLVED ANCESTOR (LEA) GFP-LIKE PROTEINS Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 26.43 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 48.773Å b: 79.018Å c: 117.894Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.151 0.195
Expression system: Escherichia coli BL21(DE3)