› 4dnx

X-ray diffraction
1.6Å resolution

The structure of the ATP sulfurylase from Allochromatium vinosum in the open state

Released:
DOI: 10.2210/pdb4dnx/pdb
PDB entry 4dnx coloured by chain, front view.
PDB entry 4dnx coloured by chain, side view.
PDB entry 4dnx coloured by chain, top view.
Source organism: Allochromatium vinosum DSM 180
Primary publication:
Structural, biochemical and genetic characterization of dissimilatory ATP sulfurylase from Allochromatium vinosum.
Parey K, Demmer U, Warkentin E, Wynen A, Ermler U, Dahl C
PLoS One 8 e74707 (2013)
PMID: 24073218

Function and Biology Details

Reaction catalysed: 
ATP + sulfate = diphosphate + adenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130293 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfate adenylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 43.8 KDa
Source organism: Allochromatium vinosum DSM 180
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66036 (Residues: 1-397; Coverage: 100%)
Gene names: Alvin_1118, sat
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 73.286Å b: 96.972Å c: 73.458Å
α: 90° β: 117.6° γ: 90°
R-values:
R R work R free
0.18 0.179 0.202
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Tyrosine sulfation as a protein post-translational modification.
Yang et al. (2015)
3 more

2 mentions without citation

Structural, biochemical and genetic characterization of dissimilatory ATP sulfurylase from Allochromatium vinosum.
Parey et al. (2013)
1 more