› 4djt

X-ray diffraction
1.8Å resolution

Crystal structure of a nuclear GTP-binding protein from Encephalitozoon cuniculi bound to GDP-Mg2+

Released:
DOI: 10.2210/pdb4djt/pdb
PDB entry 4djt coloured by chain, front view.
PDB entry 4djt coloured by chain, side view.
PDB entry 4djt coloured by chain, top view.
Source organism: Encephalitozoon cuniculi GB-M1
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding nuclear protein GSP1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 24.66 KDa
Source organism: Encephalitozoon cuniculi GB-M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8SS11 (Residues: 1-214; Coverage: 100%)
Gene names: ECU04_1560, GSP1
Sequence domains: Ras of Complex, Roc, domain of DAPkinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 42.57Å b: 89.25Å c: 54.98Å
α: 90° β: 108.06° γ: 90°
R-values:
R R work R free
0.177 0.175 0.216
Expression system: Escherichia coli

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