4di8 Summary

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PDB entry 4di8 (supersedes 4d95)

CRYSTAL STRUCTURE OF THE D248A mutant of 2-PYRONE-4,6-DICARBOXYLIC ACID HYDROLASE FROM SPHINGOMONAS PAUCIMOBILIS complexed with substrate at pH 8.5

The structure was published by Hobbs, M.E., Malashkevich, V., Williams, H.J., et al., Burley, S.K., Almo, S.C., and Raushel, F.M., in 2012 in a paper entitled "Structure and Catalytic Mechanism of LigI: Insight into the Amidohydrolase Enzymes of cog3618 and Lignin Degradation." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.81 Å and deposited in 2012.

The experimental data on which the structure is based was also deposited.

This PDB entry contains multiple copies of the structure of 2-pyrone-4,6-dicarbaxylate hydrolase.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A 2-pyrone-4,6-dicarbaxylate hydrolase O87170 (2-293) (PDCH_SPHPI)search Sphingomonas paucimobilissearch 100% 303 97%
B 2-pyrone-4,6-dicarbaxylate hydrolase O87170 (2-293) (PDCH_SPHPI)search Sphingomonas paucimobilissearch 100% 303 97%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
O87170 (2 - 293) 2-pyrone-4,6-dicarbaxylate hydrolase Sphingomonas paucimobilis

Chain Sequence family (Pfam)
A, B (O87170) PF04909: Amidohydrolasesearch

Chain ID Biological process (GO) Molecular function (GO)
A, B (O87170) metabolic processsearch protocatechuate catabolic processsearch hydrolase activitysearch 2-pyrone-4,6-dicarboxylate lactonase activitysearch catalytic activitysearch

Chain InterPro annotation
A, B Amidohydrolase 2search