4dhy Summary


Crystal structure of human glucokinase in complex with glucose and activator

The structure was published by Liu, S., Ammirati, M.J., Song, X., et al., Greasley, S.E., Pfefferkorn, J.A., and Qiu, X., in 2012 in a paper entitled "Insights into Mechanism of Glucokinase Activation: OBSERVATION OF MULTIPLE DISTINCT PROTEIN CONFORMATIONS." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.38 Å and deposited in 2012.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Glucokinase. This molecule has the UniProt identifier P35557 (HXK4_HUMAN)search. The sample contained 469 residues which is 98% of the natural sequence. Out of 469 residues 446 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Glucokinase P35557 (12-465) (HXK4_HUMAN)search Homo sapienssearch 97% 469 95%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P35557 (12 - 465) Glucokinase Homo sapiens

Chain Sequence family (Pfam)
A (P35557) PF00349: Hexokinasesearch, PF03727: Hexokinasesearch

Chain ID Biological process (GO) Cellular component (GO) Molecular function (GO)
A (P35557) metabolic processsearch glycolytic processsearch positive regulation of glycogen biosynthetic processsearch positive regulation of insulin secretionsearch glucose 6-phosphate metabolic processsearch glucose transportsearch transmembrane transportsearch negative regulation of gluconeogenesissearch glucose homeostasissearch carbohydrate metabolic processsearch endocrine pancreas developmentsearch cellular response to leptin stimulussearch positive regulation of phosphorylationsearch regulation of insulin secretionsearch glucose metabolic processsearch regulation of glycolytic processsearch fructose 2,6-bisphosphate metabolic processsearch cellular response to glucose starvationsearch cellular glucose homeostasissearch NADP metabolic processsearch calcium ion importsearch regulation of glucose transportsearch regulation of potassium ion transportsearch detection of glucosesearch cellular response to insulin stimulussearch second-messenger-mediated signalingsearch hexose transportsearch response to glucosesearch positive regulation of glycolytic processsearch phosphorylationsearch negative regulation of epinephrine secretionsearch positive regulation of cytosolic calcium ion concentrationsearch glycogen biosynthetic processsearch carbohydrate phosphorylationsearch small molecule metabolic processsearch nucleussearch cytoplasmsearch cytosolsearch mitochondrionsearch nucleoplasmsearch cell cortexsearch secretory granulesearch ATP bindingsearch phosphotransferase activity, alcohol group as acceptorsearch hexokinase activitysearch glucose bindingsearch mannokinase activitysearch glucokinase activitysearch protein phosphatase bindingsearch protein bindingsearch nucleotide bindingsearch kinase activitysearch transferase activitysearch catalytic activitysearch magnesium ion bindingsearch fructokinase activitysearch ADP bindingsearch

Chain InterPro annotation
A Hexokinasesearch Hexokinase, conserved sitesearch Hexokinase, N-terminalsearch Hexokinase, C-terminalsearch