› 4dhy

X-ray diffraction
2.38Å resolution

Crystal structure of human glucokinase in complex with glucose and activator

Released:
DOI: 10.2210/pdb4dhy/pdb
PDB entry 4dhy coloured by chain, front view.
PDB entry 4dhy coloured by chain, side view.
PDB entry 4dhy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into mechanism of glucokinase activation: observation of multiple distinct protein conformations.
Liu S, Ammirati MJ, Song X, Knafels JD, Zhang J, Greasley SE, Pfefferkorn JA, Qiu X
J Biol Chem 287 13598-610 (2012)
PMID: 22298776

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-4 Chain: A
Molecule details ›
Chain: A
Length: 469 amino acids
Theoretical weight: 52.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35557 (Residues: 12-465; Coverage: 98%)
Gene name: GCK
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLC 1 x GLC
Ligand S41 1 x S41
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 67.01Å b: 82.2Å c: 86.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.244
Expression system: Escherichia coli

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Citations

11 review citations

Solution NMR Spectroscopy for the Study of Enzyme Allostery.
Lisi et al. (2016)
10 more

3 mentions without citation

Insights into mechanism of glucokinase activation: observation of multiple distinct protein conformations.
Liu et al. (2012)
2 more