› 4dev

X-ray diffraction
2Å resolution

An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus.

Released:
DOI: 10.2210/pdb4dev/pdb
PDB entry 4dev coloured by chain, front view.
PDB entry 4dev coloured by chain, side view.
PDB entry 4dev coloured by chain, top view.
Source organism: Butyrivibrio proteoclasticus B316
Primary publication:
Structure and function of an acetyl xylan esterase (Est2A) from the rumen bacterium Butyrivibrio proteoclasticus.
Till M, Goldstone DC, Attwood GT, Moon CD, Kelly WJ, Arcus VL
Proteins 81 911-7 (2013)
PMID: 23345031

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-xylan esterase Est2A Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 408 amino acids
Theoretical weight: 46.19 KDa
Source organism: Butyrivibrio proteoclasticus B316
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E0RVY7 (Residues: 1-376; Coverage: 100%)
Gene names: bpr_I2939, est2A
Sequence domains: GDSL-like Lipase/Acylhydrolase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ACY 20 x ACY
Ligand PEG 7 x PEG
Ligand CL 1 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P1
Unit cell:
a: 90.112Å b: 95.786Å c: 98.787Å
α: 89.96° β: 99.74° γ: 92.5°
R-values:
R R work R free
0.192 0.19 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The SGNH hydrolase family: a template for carbohydrate diversity.
Anderson et al. (2022)
12 other articles

2 mentions without citation

Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass.
Kameshwar et al. (2019)
1 more