› 4dca

X-ray diffraction
1.8Å resolution

Crystal structure of aminoglycoside phosphotransferase APH(2'')-Ib, ADP-bound

Released:
DOI: 10.2210/pdb4dca/pdb
PDB entry 4dca coloured by chain, front view.
PDB entry 4dca coloured by chain, side view.
PDB entry 4dca coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Stogios PJ, Minasov G, Singer AU, Tan K, Nocek B, Evdokimova E, Egorova O, Di Leo R, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188052 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 38.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93ET9 (Residues: 1-299; Coverage: 100%)
Gene name: aph(2')-Ib
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 64.938Å b: 88.465Å c: 62.299Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.225
Expression system: Escherichia coli BL21(DE3)

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