› 4da5

X-ray diffraction
2.4Å resolution

Choline Kinase alpha acts through a double-displacement kinetic mechanism involving enzyme isomerisation, as determined through enzyme and inhibitor kinetics and structural biology

Released:
DOI: 10.2210/pdb4da5/pdb
PDB entry 4da5 coloured by chain, front view.
PDB entry 4da5 coloured by chain, side view.
PDB entry 4da5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Kinetic and mechanistic characterisation of Choline Kinase-α.
Hudson CS, Knegtel RM, Brown K, Charlton PA, Pollard JR
Biochim Biophys Acta 1834 1107-16 (2013)
PMID: 23416529

Function and Biology Details

Reactions catalysed: 
ATP + choline = ADP + phosphocholine
ATP + ethanolamine = ADP + O-phosphoethanolamine
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline kinase alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 457 amino acids
Theoretical weight: 52.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35790 (Residues: 1-457; Coverage: 100%)
Gene names: CHK, CHKA, CKI
Sequence domains: Choline/ethanolamine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand 0H7 2 x 0H7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 55.84Å b: 121.69Å c: 131.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.259
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Choline Kinase: An Unexpected Journey for a Precision Medicine Strategy in Human Diseases.
Lacal et al. (2021)
7 other articles

3 mentions without citation

New Druggable Targets for Rheumatoid Arthritis Based on Insights From Synovial Biology.
Sandhu et al. (2022)
2 more