4czv

X-ray diffraction
2.1Å resolution

Structure of the Neurospora crassa Pan2 WD40 domain

Released:

Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage of poly(A) to 5'-AMP
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-143023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAN2-PAN3 deadenylation complex catalytic subunit pan2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 36.24 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C581 (Residues: 1-321; Coverage: 25%)
Gene names: NCU04792, NCU10733, pan2, par-1
Sequence domains: PAN2 N-terminal
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 60.99Å b: 71.51Å c: 77.12Å
α: 90° β: 94.65° γ: 90°
R-values:
R R work R free
0.184 0.182 0.216
Expression system: Escherichia coli BL21(DE3)