› 4cvm

X-ray diffraction
2.06Å resolution

PaMurF in complex with AMP-PNP and UDP-MurNAc-tripeptide (mDAP)

Released:
DOI: 10.2210/pdb4cvm/pdb
PDB entry 4cvm coloured by chain, front view.
PDB entry 4cvm coloured by chain, side view.
PDB entry 4cvm coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Ruane KM, Majce V

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase Chain: A
Molecule details ›
Chain: A
Length: 475 amino acids
Theoretical weight: 49.37 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HVZ7 (Residues: 1-458; Coverage: 100%)
Gene names: PA4416, murF
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand ALA 1 x ALA
Ligand UDP 1 x UDP
Ligand MUB 1 x MUB
Ligand FGA 1 x FGA
Ligand ANP 1 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 58.54Å b: 86.05Å c: 88.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.224
Expression system: Escherichia coli

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