› 4cuf

X-ray diffraction
2.29Å resolution

Human Notch1 EGF domains 11-13 mutant T466S

Released:
DOI: 10.2210/pdb4cuf/pdb
PDB entry 4cuf coloured by chain, front view.
PDB entry 4cuf coloured by chain, side view.
PDB entry 4cuf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fringe-mediated extension of O-linked fucose in the ligand-binding region of Notch1 increases binding to mammalian Notch ligands.
Taylor P, Takeuchi H, Sheppard D, Chillakuri C, Lea SM, Haltiwanger RS, Handford PA
Proc Natl Acad Sci U S A 111 7290-5 (2014)
PMID: 24803430

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurogenic locus notch homolog protein 1 Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 14.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 411-526; Coverage: 5%)
Gene names: NOTCH1, TAN1
Sequence domains:
Structure domains: Laminin

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P6122
Unit cell:
a: 61.77Å b: 61.77Å c: 126.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.257 0.256 0.286
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The notch ligand JAGGED1 as a target for anti-tumor therapy.
Li et al. (2014)
14 more

3 mentions without citation

Fringe-mediated extension of O-linked fucose in the ligand-binding region of Notch1 increases binding to mammalian Notch ligands.
Taylor et al. (2014)
2 more