› 4cu4

X-ray diffraction
2.3Å resolution

FhuA from E. coli in complex with the lasso peptide microcin J25 (MccJ25)

Released:
DOI: 10.2210/pdb4cu4/pdb
PDB entry 4cu4 coloured by chain, front view.
PDB entry 4cu4 coloured by chain, side view.
PDB entry 4cu4 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for hijacking siderophore receptors by antimicrobial lasso peptides.
Mathavan I, Zirah S, Mehmood S, Choudhury HG, Goulard C, Li Y, Robinson CV, Rebuffat S, Beis K
Nat Chem Biol 10 340-2 (2014)
PMID: 24705590

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139319 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Ferrichrome outer membrane transporter/phage receptor Chain: A
Molecule details ›
Chain: A
Length: 706 amino acids
Theoretical weight: 78.1 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06971 (Residues: 53-438, 439-747; Coverage: 97%)
Gene names: JW0146, b0150, fhuA, tonA
Sequence domains:
Structure domains:
Microcin J25 Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.13 KDa
Source organism: Escherichia coli str. K-12 substr. MC4100
UniProt:
  • Canonical: Q9X2V7 (Residues: 38-58; Coverage: 36%)
Gene name: mcjA

Ligands and Environments

Carbohydrate polymer : NEW Components: PA1, GCS, KDO, GMH
Carbohydrate polymer
7 bound ligands:
Ligand FTT 4 x FTT
Ligand DAO 1 x DAO
Ligand MYR 1 x MYR
Ligand LDA 20 x LDA
Ligand 3PH 1 x 3PH
Ligand DPO 2 x DPO
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 95.98Å b: 277.05Å c: 106.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Bacteriocins of lactic acid bacteria: extending the family.
Alvarez-Sieiro et al. (2016)
11 more

8 mentions without citation

Mechanism of Action of Ribosomally Synthesized and Post-Translationally Modified Peptides.
Ongpipattanakul et al. (2022)
7 more