› 4csc

X-ray diffraction
1.9Å resolution

STRUCTURE OF TERNARY COMPLEXES OF CITRATE SYNTHASE WITH D-AND L-MALATE: MECHANISTIC IMPLICATIONS

Released:
DOI: 10.2210/pdb4csc/pdb
PDB entry 4csc coloured by chain, front view.
PDB entry 4csc coloured by chain, side view.
PDB entry 4csc coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
1.9-A structures of ternary complexes of citrate synthase with D- and L-malate: mechanistic implications.
Karpusas M, Holland D, Remington SJ
Biochemistry 30 6024-31 (1991)
PMID: 2043640

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149859 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate synthase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 47.32 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P23007 (Residues: 1-433; Coverage: 100%)
Gene name: CS
Sequence domains: Citrate synthase, C-terminal domain
Structure domains:

Ligands and Environments


Cofactor: Ligand ACO 1 x ACO
1 bound ligand:
Ligand MLT 1 x MLT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 104Å b: 78.1Å c: 58.3Å
α: 90° β: 78.9° γ: 90°
R-values:
R R work R free
0.188 not available not available
Expression system: Not provided

Quick links

Citations

2 review citations

Acetyl-L-carnitine physical-chemical, metabolic, and therapeutic properties: relevance for its mode of action in Alzheimer's disease and geriatric depression.
Pettegrew et al. (2000)
1 more

1 mention without citation

Multiscale analysis of enantioselectivity in enzyme-catalysed 'lethal synthesis' using projector-based embedding.
Zhang et al. (2018)