4cej Summary

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Crystal structure of ADPNP-bound AddAB bound to Chi

The structure was published by Krajewski, W.W., Fu, X., Wilkinson, M., Cronin, N.B., Dillingham, M.S., and Wigley, D.B., in 2014 in a paper entitled "Structural Basis for Translocation by Addab Helicase-Nuclease and its Arrest at Chi Sites." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.0 Å and deposited in 2013.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely ATP-DEPENDENT HELICASE/NUCLEASE SUBUNIT A, ATP-DEPENDENT HELICASE/DEOXYRIBONUCLEASE SUBUNIT B, and DNA.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A ATP-DEPENDENT HELICASE/NUCLEASE SUBUNIT A P23478 (1-1232) (ADDA_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 100% 1232 95%
B ATP-DEPENDENT HELICASE/DEOXYRIBONUCLEASE SUBUNIT B P23477 (1-1166) (ADDB_BACSU)search Bacillus subtilis subsp. subtilis str. 168search 100% 1166 99%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P23478 (1 - 1232) ATP-DEPENDENT HELICASE/NUCLEASE SUBUNIT A Bacillus subtilis subsp. subtilis str. 168
P23477 (1 - 1166) ATP-DEPENDENT HELICASE/DEOXYRIBONUCLEASE SUBUNIT B Bacillus subtilis subsp. subtilis str. 168

Chain Sequence family (Pfam)
A (P23478) PF00580: UvrD/REP helicase N-terminal domainsearch, PF12705: PD-(D/E)XK nuclease superfamilysearch, PF13361: UvrD-like helicase C-terminal domainsearch
B (P23477) PF12705: PD-(D/E)XK nuclease superfamilysearch, PF13361: UvrD-like helicase C-terminal domainsearch

Chain ID Biological process (GO) Molecular function (GO)
A (P23478) nucleic acid phosphodiester bond hydrolysissearch cellular response to DNA damage stimulussearch double-strand break repairsearch DNA duplex unwindingsearch DNA repairsearch double-strand break repair via homologous recombinationsearch nucleotide bindingsearch hydrolase activitysearch ATP-dependent DNA helicase activitysearch nuclease activitysearch ATP bindingsearch DNA bindingsearch double-stranded DNA bindingsearch 3'-5' exonuclease activitysearch exonuclease activitysearch helicase activitysearch
B (P23477) cellular response to DNA damage stimulussearch DNA repairsearch nucleic acid phosphodiester bond hydrolysissearch double-strand break repair via homologous recombinationsearch 5'-3' exonuclease activitysearch iron-sulfur cluster bindingsearch helicase activitysearch 4 iron, 4 sulfur cluster bindingsearch nucleotide bindingsearch DNA bindingsearch ATP bindingsearch hydrolase activity, acting on ester bondssearch hydrolase activitysearch exonuclease activitysearch double-stranded DNA bindingsearch nuclease activitysearch metal ion bindingsearch

Chain InterPro annotation
A DNA helicase, UvrD/REP typesearch Restriction endonuclease type II-likesearch Exonuclease, phage-type/RecB, C-terminalsearch UvrD-like Helicase, ATP-binding domainsearch DNA helicase, UvrD-like, C-terminalsearch DNA helicase subunit AddAsearch P-loop containing nucleoside triphosphate hydrolasesearch
B DNA helicase, UvrD-like, C-terminalsearch DNA helicase subunit AddBsearch P-loop containing nucleoside triphosphate hydrolasesearch