› 4c8h

X-ray diffraction
2.69Å resolution

Crystal structure of the C-terminal region of yeast Ctf4, selenomethionine protein.

Released:
DOI: 10.2210/pdb4c8h/pdb
PDB entry 4c8h coloured by chain, front view.
PDB entry 4c8h coloured by chain, side view.
PDB entry 4c8h coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome.
Simon AC, Zhou JC, Perera RL, van Deursen F, Evrin C, Ivanova ME, Kilkenny ML, Renault L, Kjaer S, Matak-Vinković D, Labib K, Costa A, Pellegrini L
Nature 510 293-297 (2014)
PMID: 24805245

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase alpha-binding protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 478 amino acids
Theoretical weight: 55.01 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01454 (Residues: 471-927; Coverage: 49%)
Gene names: CHL15, CTF4, P9659.7, POB1, YPR135W
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Unit cell:
a: 107.294Å b: 118.428Å c: 155.697Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

44 review citations

SMC complexes: from DNA to chromosomes.
Uhlmann F. (2016)
43 more

5 mentions without citation

A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome.
Simon et al. (2014)
4 more