› 4c7x

X-ray diffraction
2.29Å resolution

Thiamine Pyrophosphate Bound Transketolase from Lactobacillus salivarius at 2.2A resolution

Released:
DOI: 10.2210/pdb4c7x/pdb
PDB entry 4c7x coloured by chain, front view.
PDB entry 4c7x coloured by chain, side view.
PDB entry 4c7x coloured by chain, top view.
Source organism: Ligilactobacillus salivarius UCC118
Primary publication:
High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate.
Lukacik P, Lobley CM, Bumann M, Arena de Souza V, Owens RJ, O'Toole PW, Walsh MA
Acta Crystallogr F Struct Biol Commun 71 1327-34 (2015)
PMID: 26457526

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173080 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
transketolase Chain: A
Molecule details ›
Chain: A
Length: 681 amino acids
Theoretical weight: 74.54 KDa
Source organism: Ligilactobacillus salivarius UCC118
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1WQU8 (Residues: 2-663; Coverage: 100%)
Gene names: LSL_1946, tktA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 1 x TPP
1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P3221
Unit cell:
a: 74.99Å b: 74.99Å c: 192.85Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.154 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate.
Lukacik et al. (2015)