› 4bxo

X-ray diffraction
2.15Å resolution

Architecture and DNA recognition elements of the Fanconi anemia FANCM- FAAP24 complex

Released:
DOI: 10.2210/pdb4bxo/pdb
PDB entry 4bxo coloured by chain, front view.
PDB entry 4bxo coloured by chain, side view.
PDB entry 4bxo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Architecture and DNA Recognition Elements of the Fanconi Anemia FANCM-FAAP24 Complex.
Coulthard R, Deans AJ, Swuec P, Bowles M, Costa A, West SC, McDonald NQ
Structure (2013)
PMID: 23932590

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117266 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Fanconi anemia group M protein Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IYD8 (Residues: 1798-2048; Coverage: 12%)
Gene names: FANCM, KIAA1596
Sequence domains: ERCC4 domain
Structure domains:
Fanconi anemia core complex-associated protein 24 Chain: B
Molecule details ›
Chain: B
Length: 217 amino acids
Theoretical weight: 24.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BTP7 (Residues: 1-214; Coverage: 100%)
Gene names: C19orf40, FAAP24
Sequence domains:
Structure domains:
5'-D(*TP*CP*AP*GP*CP*AP*TP*CP*AP*TP*CP)-3' Chain: H
Molecule details ›
Chain: H
Length: 11 nucleotides
Theoretical weight: 3.41 KDa
5'-D(*GP*AP*TP*GP*AP*TP*GP*CP*TP*GP*CP)-3' Chain: I
Molecule details ›
Chain: I
Length: 11 nucleotides
Theoretical weight: 3.29 KDa

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P41212
Unit cell:
a: 125.1Å b: 125.1Å c: 74.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The Fanconi anemia DNA repair pathway: structural and functional insights into a complex disorder.
Walden et al. (2014)
9 more

1 mention without citation

A meiotic XPF-ERCC1-like complex recognizes joint molecule recombination intermediates to promote crossover formation.
De Muyt et al. (2018)