› 4bwx

X-ray diffraction
2.85Å resolution

Structure of Neurospora crassa PAN3 pseudokinase mutant

Released:
DOI: 10.2210/pdb4bwx/pdb
PDB entry 4bwx coloured by chain, front view.
PDB entry 4bwx coloured by chain, side view.
PDB entry 4bwx coloured by chain, top view.
Source organism: Neurospora crassa
Primary publication:
Structure of the PAN3 pseudokinase reveals the basis for interactions with the PAN2 deadenylase and the GW182 proteins.
Christie M, Boland A, Huntzinger E, Weichenrieder O, Izaurralde E
Mol Cell 51 360-73 (2013)
PMID: 23932717

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181923 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAN2-PAN3 deadenylation complex subunit pan3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 49.38 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7SDP4 (Residues: 234-656; Coverage: 65%)
Gene names: NCU01929, pan3, par-2
Sequence domains: Pan3 Pseudokinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AGS 2 x AGS
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P65
Unit cell:
a: 90.312Å b: 90.312Å c: 230.609Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.211 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

28 review citations

Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation.
O'Brien et al. (2018)
27 more

1 mention without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)