spacer rat NTPDase2 in complex with Ca AMPNP
Accession O35795search
Keywords Magnesium, Cell membrane, 3D-structure, Glycoprotein, Nucleotide-binding, Membrane, Transmembrane, ATP-binding, Disulfide bond, Reference proteome, Hydrolase, Alternative splicing, Calcium, Complete proteome, Transmembrane helix
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Polymers A
Enzyme nomenclature
EC number 3.6.1.- ExPASy BRENDA search
Polymers A
EC number ExPASy BRENDA search
Polymers A
Name apyrase, apyrase
Comment Requires Ca2+. Also acts on ADP, and on other nucleoside triphosphates and diphosphates. Most of the ecto-ATPases occurring on the cell surface and hydrolysing extracellular nucleoside triphosphates and diphosphates belong to this enzyme family. Either Ca2+ or Mg2+ can serve as activating ions.
Apyrases are active against both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyse NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates. They differ from ATPases, which specifically hydrolyse ATP, by hydrolysing both ATP and ADP. The eukaryotic enzymes requires Ca2+, but Mg2+ can substitute. Most of the ecto-ATPases that occur on the cell surface and hydrolyse extracellular nucleotides belong to this enzyme family.