› 4bpl

X-ray diffraction
2.3Å resolution

rice importin_alpha in complex with nucleoplasmin NLS

Released:
DOI: 10.2210/pdb4bpl/pdb
PDB entry 4bpl coloured by chain, front view.
PDB entry 4bpl coloured by chain, side view.
PDB entry 4bpl coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of interaction of bipartite nuclear localization signal from Agrobacterium VirD2 with rice importin-α.
Chang CW, Williams SJ, Couñago RM, Kobe B
Mol Plant 7 1061-1064 (2014)
PMID: 24503158

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138601 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1a Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 49.24 KDa
Source organism: Oryza sativa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q71VM4 (Residues: 73-526; Coverage: 86%)
Gene names: LOC_Os01g14950, Os01g0253300
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Nucleoplasmin Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.23 KDa
Source organism: Xenopus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05221 (Residues: 152-172; Coverage: 11%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 62.221Å b: 74.093Å c: 138.027Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
6 other articles