› 4bmx

X-ray diffraction
1.76Å resolution

Native structure of futalosine hydrolase of Helicobacter pylori strain 26695

Released:
DOI: 10.2210/pdb4bmx/pdb
PDB entry 4bmx coloured by chain, front view.
PDB entry 4bmx coloured by chain, side view.
PDB entry 4bmx coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Structural enzymology of Helicobacter pylori methylthioadenosine nucleosidase in the futalosine pathway.
Kim RQ, Offen WA, Davies GJ, Stubbs KA
Acta Crystallogr D Biol Crystallogr 70 177-85 (2014)
PMID: 24419390

Function and Biology Details

Reactions catalysed: 
6-amino-6-deoxyfutalosine + H(2)O = dehypoxanthine futalosine + adenine
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminodeoxyfutalosine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 27.41 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O24915 (Residues: 1-231; Coverage: 100%)
Gene names: HP_0089, mtn, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand TRS 2 x TRS
Ligand ADE 1 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 59.314Å b: 90.573Å c: 108.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.212
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Biosynthesis and applications of prenylquinones.
Kawamukai M. (2018)
5 other articles