4bl0 Summary

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Crystal structure of yeast Bub3-Bub1 bound to phospho-Spc105

The structure was published by Primorac, I., Weir, J.R., Chiroli, E., et al., Van Gerwen, S., Ciliberto, A., and Musacchio, A., in 2013 in a paper entitled "Bub3 Reads Phosphorylated Melt Repeats to Promote Spindle Assembly Checkpoint Signaling" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.95 Å and deposited in 2013.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely CELL CYCLE ARREST PROTEIN BUB3, CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1, and SPINDLE POLE BODY COMPONENT SPC105.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A CELL CYCLE ARREST PROTEIN BUB3 P26449 (1-341) (BUB3_YEAST)search Saccharomyces cerevisiae S288csearch 100% 341 97%
D CELL CYCLE ARREST PROTEIN BUB3 P26449 (1-341) (BUB3_YEAST)search Saccharomyces cerevisiae S288csearch 100% 341 97%
B CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 P41695 (289-359) (BUB1_YEAST)search Saccharomyces cerevisiae S288csearch < 90% 75 60%
E CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 P41695 (289-359) (BUB1_YEAST)search Saccharomyces cerevisiae S288csearch < 90% 75 60%
C SPINDLE POLE BODY COMPONENT SPC105 P53148 (165-183) (SP105_YEAST)search Saccharomyces cerevisiae S288csearch < 90% 19 63%
F SPINDLE POLE BODY COMPONENT SPC105 P53148 (165-183) (SP105_YEAST)search Saccharomyces cerevisiae S288csearch < 90% 19 63%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P26449 (1 - 341) CELL CYCLE ARREST PROTEIN BUB3 Saccharomyces cerevisiae
P41695 (289 - 359) CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 Saccharomyces cerevisiae
P53148 (165 - 183) SPINDLE POLE BODY COMPONENT SPC105 SACCHAROMYCES CEREVISIAE

Chain Sequence family (Pfam)
A, D (P26449) PF00400: WD domain, G-beta repeatsearch
B, E Mad3/BUB1 homology region 2search
C, F

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, D (P26449) mitotic spindle assembly checkpointsearch cell cyclesearch mitotic DNA integrity checkpointsearch protein bindingsearch ubiquitin bindingsearch nucleussearch mitotic checkpoint complexsearch condensed nuclear chromosome kinetochoresearch
B, E (P41695) cell cycle checkpointsearch nucleussearch

Chain InterPro annotation
A, D WD40 repeatsearch WD40/YVTN repeat-like-containing domainsearch WD40-repeat-containing domainsearch
B, E Mad3-likesearch
C, F