› 4beq

X-ray diffraction
1.5Å resolution

Structure of Vibrio cholerae broad spectrum racemase double mutant R173A, N174A

Released:
DOI: 10.2210/pdb4beq/pdb
PDB entry 4beq coloured by chain, front view.
PDB entry 4beq coloured by chain, side view.
PDB entry 4beq coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Structural basis for the broad specificity of a new family of amino-acid racemases.
Espaillat A, Carrasco-López C, Bernardo-García N, Pietrosemoli N, Otero LH, Álvarez L, de Pedro MA, Pazos F, Davis BM, Waldor MK, Hermoso JA, Cava F
Acta Crystallogr D Biol Crystallogr 70 79-90 (2014)
PMID: 24419381

Function and Biology Details

Reaction catalysed: 
An L-amino acid = a D-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Broad specificity amino-acid racemase Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 41.76 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KSE5 (Residues: 24-407; Coverage: 100%)
Gene names: VC_1312, bsrV
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 95.997Å b: 50.99Å c: 76.455Å
α: 90° β: 100.69° γ: 90°
R-values:
R R work R free
0.149 0.147 0.17
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Bacterial synthesis of D-amino acids.
Radkov et al. (2014)
11 more

2 mentions without citation

Structural basis for the broad specificity of a new family of amino-acid racemases.
Espaillat et al. (2014)
1 more