› 4be4

X-ray diffraction
2.6Å resolution

Closed conformation of O. piceae sterol esterase

Released:
DOI: 10.2210/pdb4be4/pdb
PDB entry 4be4 coloured by chain, front view.
PDB entry 4be4 coloured by chain, side view.
PDB entry 4be4 coloured by chain, top view.
Source organism: Ophiostoma piceae
Primary publication:
Crystal structures of Ophiostoma piceae sterol esterase: structural insights into activation mechanism and product release.
Gutiérrez-Fernández J, Vaquero ME, Prieto A, Barriuso J, Martínez MJ, Hermoso JA
J Struct Biol 187 215-222 (2014)
PMID: 25108239

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylic ester hydrolase Chain: A
Molecule details ›
Chain: A
Length: 545 amino acids
Theoretical weight: 59.21 KDa
Source organism: Ophiostoma piceae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q2TFW1 (Residues: 13-549; Coverage: 98%)
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

5 bound ligands:
Ligand NAG 2 x NAG
Ligand DIO 6 x DIO
Ligand MES 1 x MES
Ligand GOL 6 x GOL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6322
Unit cell:
a: 119.274Å b: 119.274Å c: 206.774Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.164 0.221
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Structural traits and catalytic versatility of the lipases from the Candida rugosa-like family: A review.
Barriuso et al. (2016)
1 more

2 mentions without citation

Evolutionary history of versatile-lipases from Agaricales through reconstruction of ancestral structures.
Barriuso et al. (2017)
1 more