› 4bcc

X-ray diffraction
1.65Å resolution

PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN WITH A COVALENTLY BOUND P2- substituted N-acyl-prolylpyrrolidine inhibitor

Released:
DOI: 10.2210/pdb4bcc/pdb
PDB entry 4bcc coloured by chain, front view.
PDB entry 4bcc coloured by chain, side view.
PDB entry 4bcc coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
P2-substituted N-acylprolylpyrrolidine inhibitors of prolyl oligopeptidase: biochemical evaluation, binding mode determination, and assessment in a cellular model of synucleinopathy.
Van der Veken P, Fülöp V, Rea D, Gerard M, Van Elzen R, Joossens J, Cheng JD, Baekelandt V, De Meester I, Lambeir AM, Augustyns K
J Med Chem 55 9856-67 (2012)
PMID: 23121075

Function and Biology Details

Reaction catalysed: 
Hydrolysis of --Pro-|- and to a lesser extent --Ala-|- in oligopeptides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prolyl endopeptidase Chain: A
Molecule details ›
Chain: A
Length: 710 amino acids
Theoretical weight: 80.86 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P23687 (Residues: 1-710; Coverage: 100%)
Gene name: PREP
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand JKT 1 x JKT
Ligand TAM 1 x TAM
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 70.49Å b: 99.14Å c: 110.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.19
Expression system: Escherichia coli

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Citations

1 review citation

Peptide POP inhibitors for the treatment of the cognitive symptoms of schizophrenia.
López et al. (2013)
12 other articles

2 mentions without citation

Optical design and performance of the biological small-angle X-ray scattering beamline at the Taiwan Photon Source.
Liu et al. (2021)
1 more