› 4b7c

X-ray diffraction
2.1Å resolution

Crystal structure of hypothetical protein PA1648 from Pseudomonas aeruginosa.

Released:
DOI: 10.2210/pdb4b7c/pdb
PDB entry 4b7c coloured by chain, front view.
PDB entry 4b7c coloured by chain, side view.
PDB entry 4b7c coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery.
Moynie L, Schnell R, McMahon SA, Sandalova T, Boulkerou WA, Schmidberger JW, Alphey M, Cukier C, Duthie F, Kopec J, Liu H, Jacewicz A, Hunter WN, Naismith JH, Schneider G
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 25-34 (2013)
PMID: 23295481

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl reductase (ER) domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 336 amino acids
Theoretical weight: 36.33 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I377 (Residues: 1-334; Coverage: 100%)
Gene name: PA1648
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MES 5 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 170.93Å b: 177.09Å c: 181.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.223 0.243
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Lytic transglycosylases: concinnity in concision of the bacterial cell wall.
Dik et al. (2017)
3 more

1 mention without citation

The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery.
Moynie et al. (2013)