Structure analysis

Crystal structure of the 30S ribosome in complex with compound 37

X-ray diffraction
3.15Å resolution
Source organism: Thermus thermophilus HB8
Assembly composition:
hetero 23-mer (preferred)
Entry contents: 20 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 23-mer
Accessible surface area: 274978.59 Å2
Buried surface area: 113984.85 Å2
Dissociation area: 1,600.53 Å2
Dissociation energy (ΔGdiss): 14.47 kcal/mol
Dissociation entropy (TΔSdiss): 12.69 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-215906

Macromolecules

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Chain: H
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY9 (Residues: 1-138; Coverage: 100%)
Gene names: TTHA1678, rpsH
Pfam: Ribosomal protein S8
InterPro:
CATH:

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Chain: L
Length: 132 amino acids
Theoretical weight: 14.64 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:
CATH: Nucleic acid-binding proteins

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Chain: O
Length: 88 amino acids
Theoretical weight: 10.45 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ76 (Residues: 2-89; Coverage: 99%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
InterPro:
CATH: S15/NS1, RNA-binding

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Chain: P
Length: 88 amino acids
Theoretical weight: 10.41 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
InterPro:
CATH: S16 Ribosomal Protein; Chain: A;

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Chain: R
Length: 88 amino acids
Theoretical weight: 10.24 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
InterPro:
CATH: Ribosomal protein S18

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Chain: T
Length: 106 amino acids
Theoretical weight: 11.72 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80380 (Residues: 1-106; Coverage: 100%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
InterPro:
CATH: Ribosomal protein S20

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Chain: V
Length: 26 amino acids
Theoretical weight: 3.22 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SIH3 (Residues: 2-27; Coverage: 96%)
Gene names: TTHA1396, rpsU
Pfam: 30S ribosomal protein Thx
InterPro:

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Name: 16S ribosomal RNA
Representative chains: A
Length: 1521 nucleotides
Theoretical weight: 493.65 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: 5'-R(*UP*UP*CP*AP*AP*AP)-3'
Representative chains: W
Length: 6 nucleotides
Theoretical weight: 1.86 KDa

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Name: 5'-R(*GP*GP*GP*AP*UP*UP*GP*AP*AP*AP*AP*UP*CP*CP*CP)-3'
Representative chains: Z
Length: 16 nucleotides
Theoretical weight: 5.16 KDa

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