4atd

X-ray diffraction
2.1Å resolution

Crystal structure of native Raucaffricine glucosidase

Released:
Source organism: Rauvolfia serpentina
Primary publication:
High speed X-ray analysis of plant enzymes at room temperature.
Phytochemistry 91 88-92 (2013)
PMID: 22704651

Function and Biology Details

Reactions catalysed:
UDP-glucose + vomilenine = UDP + raucaffricine
Raucaffricine + H(2)O = D-glucose + vomilenine
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-193499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Raucaffricine-O-beta-D-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 57.83 KDa
Source organism: Rauvolfia serpentina
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SPP9 (Residues: 1-513; Coverage: 95%)
Gene names: RG, VGT
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I222
Unit cell:
a: 102.237Å b: 127.253Å c: 216.111Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.206
Expression system: Escherichia coli