› 4asw

Solution NMR

Structure of the complex between the N-terminal dimerisation domain of Sgt2 and the UBL domain of Get5

Released:
DOI: 10.2210/pdb4asw/pdb
PDB entry 4asw coloured by chain, ensemble of 9 models, front view.
PDB entry 4asw coloured by chain, ensemble of 9 models, side view.
PDB entry 4asw coloured by chain, ensemble of 9 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the Sgt2/Get5 complex provides insights into GET-mediated targeting of tail-anchored membrane proteins.
Simon AC, Simpson PJ, Goldstone RM, Krysztofinska EM, Murray JW, High S, Isaacson RL
Proc Natl Acad Sci U S A 110 1327-32 (2013)
PMID: 23297211

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171298 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Small glutamine-rich tetratricopeptide repeat-containing protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 92 amino acids
Theoretical weight: 10.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12118 (Residues: 1-78; Coverage: 23%)
Gene names: SGT2, UNF346, YOR007C
Sequence domains: Homodimerisation domain of SGTA
Structure domains: Immunoglobulin FC, subunit C
Ubiquitin-like protein MDY2 Chain: C
Molecule details ›
Chain: C
Length: 83 amino acids
Theoretical weight: 9.27 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12285 (Residues: 70-152; Coverage: 39%)
Gene names: GET5, MDY2, TMA24, YOL111C
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 30%
Refinement method: ARIA
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Mechanisms of Tail-Anchored Membrane Protein Targeting and Insertion.
Chio et al. (2017)
7 more

1 mention without citation

Structure of the Sgt2/Get5 complex provides insights into GET-mediated targeting of tail-anchored membrane proteins.
Simon et al. (2013)