› 4aso

X-ray diffraction
7Å resolution

TubR bound to 24 bp of tubC from Bacillus thuringiensis serovar israelensis pBtoxis

Released:
DOI: 10.2210/pdb4aso/pdb
PDB entry 4aso coloured by chain, front view.
PDB entry 4aso coloured by chain, side view.
PDB entry 4aso coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
Superstructure of the centromeric complex of TubZRC plasmid partitioning systems.
Aylett CH, Löwe J
Proc Natl Acad Sci U S A 109 16522-7 (2012)
PMID: 23010931

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117219 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding transcriptional repressor TubR Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 104 amino acids
Theoretical weight: 12.12 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8KNP2 (Residues: 1-104; Coverage: 100%)
Gene names: ATN07_33545, pBt157, tubR
TUBC FROM BACILLUS THURINGIENSIS PBTOXIS 24 BP Chains: S, U, W, Y
Molecule details ›
Chains: S, U, W, Y
Length: 24 nucleotides
Theoretical weight: 7.32 KDa
Source organism: Bacillus thuringiensis
Expression system: Not provided
TUBC FROM BACILLUS THURINGIENSIS PBTOXIS 24 BP Chains: T, V, X, Z
Molecule details ›
Chains: T, V, X, Z
Length: 24 nucleotides
Theoretical weight: 7.41 KDa
Source organism: Bacillus thuringiensis
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 96 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 519.251Å b: 63.657Å c: 167.263Å
α: 90° β: 96.66° γ: 90°
R-values:
R R work R free
0.346 0.344 0.371
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

4 review citations

Bacterial Filament Systems: Toward Understanding Their Emergent Behavior and Cellular Functions.
Eun et al. (2015)
3 more

3 mentions without citation

Superstructure of the centromeric complex of TubZRC plasmid partitioning systems.
Aylett et al. (2012)
2 more