4aqv Summary

pdbe.org/4aqv
spacer

Model of human kinesin-5 motor domain (3HQD) and mammalian tubulin heterodimer (1JFF) docked into the 9.7-angstrom cryo-EM map of microtubule-bound kinesin-5 motor domain in the AMPPPNP state.

The structure was published by Goulet, A., Behnke-Parks, W.M., Sindelar, C.V., Major, J., Rosenfeld, S.S., and Moores, C., in 2012 in a paper entitled "The Structural Basis of Force Generation by the Mitotic Motor Kinesin-5." (abstract).

The structure was determined using Electron microscopy at a resolution of 9.7 Å and deposited in 2012.

The experimental data on which the structure is based was deposited separately in EMDB as entry 2077.

This PDB entry contains a complex of 3 biomacromolecules, namely TUBULIN ALPHA-1D CHAIN, TUBULIN BETA-2B CHAIN, and KINESIN-LIKE PROTEIN KIF11.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A TUBULIN ALPHA-1D CHAIN Q2HJ86 (1-452) (TBA1D_BOVIN)search Bos taurussearch 97% 452 91%
B TUBULIN BETA-2B CHAIN Q6B856 (1-54) (TBB2B_BOVIN)search ,
Q6B856 (56-169) (TBB2B_BOVIN)search ,
Q6B856 (171-295) (TBB2B_BOVIN)search ,
Q6B856 (297-315) (TBB2B_BOVIN)search ,
Q6B856 (317-445) (TBB2B_BOVIN)search
Bos taurussearch ,
Bos taurussearch ,
Bos taurussearch ,
Bos taurussearch ,
Bos taurussearch
96% ,
96% ,
96% ,
96% ,
96%
445 95%
C KINESIN-LIKE PROTEIN KIF11 P52732 (1-367) (KIF11_HUMAN)search Homo sapienssearch < 90% 373 96%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
Q2HJ86 (1 - 452) TUBULIN ALPHA-1D CHAIN Bos taurus
Q6B856 (1 - 54) TUBULIN BETA-2B CHAIN Bos taurus
P52732 (1 - 367) KINESIN-LIKE PROTEIN KIF11 Homo sapiens

Chain Sequence family (Pfam)
A (Q2HJ86) PF00091: Tubulin/FtsZ family, GTPase domainsearch, PF03953: Tubulin C-terminal domainsearch
B (Q6B856) PF00091: Tubulin/FtsZ family, GTPase domainsearch, PF03953: Tubulin C-terminal domainsearch
C Kinesin motor domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (Q2HJ86) GTP catabolic processsearch microtubule-based processsearch protein polymerizationsearch GTP bindingsearch GTPase activitysearch nucleotide bindingsearch structural constituent of cytoskeletonsearch microtubulesearch protein complexsearch cytoskeletonsearch cytoplasmsearch
B (Q6B856) GTP catabolic processsearch microtubule-based processsearch protein polymerizationsearch GTP bindingsearch GTPase activitysearch structural constituent of cytoskeletonsearch protein complexsearch microtubulesearch
C (P52732) microtubule-based movementsearch microtubule motor activitysearch ATP bindingsearch microtubule bindingsearch kinesin complexsearch

Chain InterPro annotation
A Tubulinsearch Alpha tubulinsearch Tubulin/FtsZ, GTPase domainsearch Tubulin/FtsZ, C-terminalsearch Tubulin, conserved sitesearch Tubulin/FtsZ, 2-layer sandwich domainsearch Tubulin, C-terminalsearch
B Tubulinsearch Beta tubulinsearch Tubulin/FtsZ, GTPase domainsearch Tubulin/FtsZ, C-terminalsearch Beta tubulin, autoregulation binding sitesearch Tubulin, conserved sitesearch Tubulin/FtsZ, 2-layer sandwich domainsearch Tubulin, C-terminalsearch
C Kinesin, motor domainsearch Kinesin, motor region, conserved sitesearch P-loop containing nucleoside triphosphate hydrolasesearch Kinesin-like proteinsearch