› 4al2

X-ray diffraction
2.6Å resolution

peptide deformylase (Ni-form) with hydrosulfide

Released:
DOI: 10.2210/pdb4al2/pdb
PDB entry 4al2 coloured by chain, front view.
PDB entry 4al2 coloured by chain, side view.
PDB entry 4al2 coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
A FRET enzyme-based probe for monitoring hydrogen sulfide.
Strianese M, Palm GJ, Milione S, Kühl O, Hinrichs W, Pellecchia C
Inorg Chem 51 11220-2 (2012)
PMID: 23072298

Function and Biology Details

Reaction catalysed: 
Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide deformylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 186 amino acids
Theoretical weight: 21.23 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6K3 (Residues: 2-169; Coverage: 99%)
Gene names: JW3248, b3287, def, fms
Sequence domains: Polypeptide deformylase
Structure domains: Peptide deformylase

Ligands and Environments

2 bound ligands:
Ligand NI 3 x NI
Ligand H2S 3 x H2S
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 142.206Å b: 63.222Å c: 118.175Å
α: 90° β: 144.28° γ: 90°
R-values:
R R work R free
0.211 0.206 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Thiol reactive probes and chemosensors.
Peng et al. (2012)
3 more