› 4ak0

X-ray diffraction
2.28Å resolution

Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin

Released:
DOI: 10.2210/pdb4ak0/pdb
PDB entry 4ak0 coloured by chain, front view.
PDB entry 4ak0 coloured by chain, side view.
PDB entry 4ak0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ligand-controlled assembly of hexamers, dihexamers, and linear multihexamer structures by the engineered acylated insulin degludec.
Steensgaard DB, Schluckebier G, Strauss HM, Norrman M, Thomsen JK, Friderichsen AV, Havelund S, Jonassen I
Biochemistry 52 295-309 (2013)
PMID: 23256685

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134210 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 29 amino acids
Theoretical weight: 3.33 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-53; Coverage: 34%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: I213
Unit cell:
a: 77.8Å b: 77.8Å c: 77.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.166 0.232
Expression system: Saccharomyces cerevisiae

Quick links

Citations

17 review citations

A review of the pharmacological properties of insulin degludec and their clinical relevance.
Haahr et al. (2014)
16 more

1 mention without citation

How Structural Biologists and the Protein Data Bank Contributed to Recent FDA New Drug Approvals.
Westbrook et al. (2019)