› 4aek

X-ray diffraction
1.75Å resolution

Structural and biochemical characterization of a novel Carbohydrate Binding Module of endoglucanase Cel5A from Eubacterium cellulosolvens

Released:
DOI: 10.2210/pdb4aek/pdb
PDB entry 4aek coloured by chain, front view.
PDB entry 4aek coloured by chain, side view.
PDB entry 4aek coloured by chain, top view.
Source organism: [Eubacterium] cellulosolvens
Primary publication:
Understanding how noncatalytic carbohydrate binding modules can display specificity for xyloglucan.
Luís AS, Venditto I, Temple MJ, Rogowski A, Baslé A, Xue J, Knox JP, Prates JA, Ferreira LM, Fontes CM, Najmudin S, Gilbert HJ
J Biol Chem 288 4799-809 (2013)
PMID: 23229556

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174729 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase cel5A Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 14.88 KDa
Source organism: [Eubacterium] cellulosolvens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3LHN3 (Residues: 37-170; Coverage: 12%)
Gene name: cel5A
Sequence domains: Carbohydrate binding module 65 domain 1
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6122
Unit cell:
a: 47.859Å b: 47.859Å c: 191.598Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.227
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules.
Gilbert et al. (2013)
2 more

3 mentions without citation

Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass.
Kameshwar et al. (2019)
2 more