› 4a94

X-ray diffraction
1.7Å resolution

Structure of the carboxypeptidase inhibitor from Nerita versicolor in complex with human CPA4

Released:
DOI: 10.2210/pdb4a94/pdb
PDB entry 4a94 coloured by chain, front view.
PDB entry 4a94 coloured by chain, side view.
PDB entry 4a94 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of novel metallocarboxypeptidase inhibitor from marine mollusk Nerita versicolor in complex with human carboxypeptidase A4.
Covaleda G, del Rivero MA, Chávez MA, Avilés FX, Reverter D
J Biol Chem 287 9250-8 (2012)
PMID: 22294694

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160987 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carboxypeptidase A4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 310 amino acids
Theoretical weight: 34.85 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9UI42 (Residues: 112-421; Coverage: 77%)
Gene names: CPA3, CPA4, UNQ694/PRO1339
Sequence domains: Zinc carboxypeptidase
Structure domains: Zn peptidases
Metallocarboxypeptidase inhibitor Chains: C, D
Molecule details ›
Chains: C, D
Length: 53 amino acids
Theoretical weight: 5.96 KDa
Source organism: Nerita versicolor
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P86912 (Residues: 1-53; Coverage: 100%)
Structure domains: Laminin

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand NO3 6 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 69.22Å b: 71.98Å c: 79.84Å
α: 90° β: 108.84° γ: 90°
R-values:
R R work R free
0.207 0.205 0.235
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Marine Invertebrates: A Promissory Still Unexplored Source of Inhibitors of Biomedically Relevant Metallo Aminopeptidases Belonging to the M1 and M17 Families.
Pascual Alonso et al. (2023)
12 other articles

4 mentions without citation

Crystal structure of novel metallocarboxypeptidase inhibitor from marine mollusk Nerita versicolor in complex with human carboxypeptidase A4.
Covaleda et al. (2012)
3 more