4a4c Summary

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Structure of phosphoTyr371-c-Cbl-UbcH5B-ZAP-70 complex

There is a Quite Interesting Protein Structure article for this entry.

The structure was published by Dou, H., Buetow, L., Hock, A., Sibbet, G.J., Vousden, K.H., and Huang, D.T., in 2012 in a paper entitled "Structural Basis for Autoinhibition and Phosphorylation-Dependent Activation of C-Cbl" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.704 Å and deposited in 2011.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely E3 UBIQUITIN-PROTEIN LIGASE CBL, TYROSINE-PROTEIN KINASE ZAP-70, and UBIQUITIN-CONJUGATING ENZYME E2 D2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A E3 UBIQUITIN-PROTEIN LIGASE CBL P22681 (47-435) (CBL_HUMAN)search Homo sapienssearch < 90% 391 99%
B TYROSINE-PROTEIN KINASE ZAP-70 P43403 (286-297) (ZAP70_HUMAN)search Homo sapienssearch 98% 12 75%
C UBIQUITIN-CONJUGATING ENZYME E2 D2 P62837 (1-147) (UB2D2_HUMAN)search Homo sapienssearch 99% 147 98%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P22681 (47 - 435) E3 UBIQUITIN-PROTEIN LIGASE CBL Homo sapiens
P43403 (286 - 297) TYROSINE-PROTEIN KINASE ZAP-70 HOMO SAPIENS
P62837 (1 - 147) UBIQUITIN-CONJUGATING ENZYME E2 D2 Homo sapiens

Chain Sequence family (Pfam)
A Zinc finger, C3HC4 type (RING finger)search, CBL proto-oncogene N-terminal domain 1search, CBL proto-oncogene N-terminus, EF hand-like domainsearch, CBL proto-oncogene N-terminus, SH2-like domainsearch
B (P43403)
C (P62837) PF00179: Ubiquitin-conjugating enzymesearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (P22681) phosphotyrosine bindingsearch signal transducer activitysearch calcium ion bindingsearch zinc ion bindingsearch ubiquitin-protein transferase activitysearch cell surface receptor signaling pathwaysearch regulation of signalingsearch nucleussearch
C (P62837) acid-amino acid ligase activitysearch protein bindingsearch ubiquitin-protein transferase activitysearch ligase activitysearch ATP bindingsearch nucleotide bindingsearch toll-like receptor 4 signaling pathwaysearch cellular protein modification processsearch MyD88-independent toll-like receptor signaling pathwaysearch toll-like receptor signaling pathwaysearch protein ubiquitinationsearch protein K48-linked ubiquitinationsearch cellular response to hypoxiasearch toll-like receptor 3 signaling pathwaysearch TRIF-dependent toll-like receptor signaling pathwaysearch protein polyubiquitinationsearch ubiquitin-dependent protein catabolic processsearch regulation of transcription from RNA polymerase II promoter in response to hypoxiasearch innate immune responsesearch nucleoplasmsearch cytosolsearch ubiquitin ligase complexsearch extracellular vesicular exosomesearch protein complexsearch

Chain InterPro annotation
A SH2 domainsearch Zinc finger, RING-typesearch Adaptor protein Cbl, N-terminal helicalsearch EF-hand domain pairsearch Zinc finger, RING/FYVE/PHD-typesearch Adaptor protein Cbl, EF hand-likesearch Adaptor protein Cbl, SH2-likesearch Zinc finger, RING-type, conserved sitesearch Adaptor protein Cbl, PTB domainsearch Adaptor protein Cblsearch
B
C Ubiquitin-conjugating enzyme E2search Ubiquitin-conjugating enzyme/RWD-likesearch Ubiquitin-conjugating enzyme, active sitesearch