4a4c Summary


Structure of phosphoTyr371-c-Cbl-UbcH5B-ZAP-70 complex

There is a Quite Interesting Protein Structure article for this entry.

The structure was published by Dou, H., Buetow, L., Hock, A., Sibbet, G.J., Vousden, K.H., and Huang, D.T., in 2012 in a paper entitled "Structural Basis for Autoinhibition and Phosphorylation-Dependent Activation of C-Cbl" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.704 Å and deposited in 2011.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely E3 UBIQUITIN-PROTEIN LIGASE CBL, TYROSINE-PROTEIN KINASE ZAP-70, and UBIQUITIN-CONJUGATING ENZYME E2 D2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A E3 UBIQUITIN-PROTEIN LIGASE CBL P22681 (47-435) (CBL_HUMAN)search Homo sapienssearch < 90% 391 99%
B TYROSINE-PROTEIN KINASE ZAP-70 P43403 (286-297) (ZAP70_HUMAN)search Homo sapienssearch 98% 12 75%
C UBIQUITIN-CONJUGATING ENZYME E2 D2 P62837 (1-147) (UB2D2_HUMAN)search Homo sapienssearch 99% 147 98%

This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P22681 (47 - 435) E3 UBIQUITIN-PROTEIN LIGASE CBL Homo sapiens
P62837 (1 - 147) UBIQUITIN-CONJUGATING ENZYME E2 D2 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A Zinc finger, C3HC4 type (RING finger)search, CBL proto-oncogene N-terminal domain 1search, CBL proto-oncogene N-terminus, EF hand-like domainsearch, CBL proto-oncogene N-terminus, SH2-like domainsearch
B (P43403)
C (P62837) Ubiquitin Conjugating Enzymesearch PF00179: Ubiquitin-conjugating enzymesearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (P22681) cell surface receptor signaling pathwaysearch regulation of signalingsearch zinc ion bindingsearch signal transducer activitysearch phosphotyrosine bindingsearch ubiquitin-protein transferase activitysearch calcium ion bindingsearch nucleussearch
C (P62837) protein ubiquitinationsearch ubiquitin-dependent protein catabolic processsearch protein polyubiquitinationsearch cellular protein modification processsearch regulation of transcription from RNA polymerase II promoter in response to hypoxiasearch TRIF-dependent toll-like receptor signaling pathwaysearch cellular response to hypoxiasearch toll-like receptor 3 signaling pathwaysearch protein K48-linked ubiquitinationsearch toll-like receptor signaling pathwaysearch toll-like receptor 4 signaling pathwaysearch MyD88-independent toll-like receptor signaling pathwaysearch innate immune responsesearch ubiquitin conjugating enzyme activitysearch protein bindingsearch ubiquitin-protein transferase activitysearch ligase activitysearch acid-amino acid ligase activitysearch nucleotide bindingsearch ATP bindingsearch cytosolsearch protein complexsearch nucleoplasmsearch ubiquitin ligase complexsearch extracellular vesicular exosomesearch

Chain InterPro annotation
A SH2 domainsearch Zinc finger, RING-typesearch Adaptor protein Cbl, N-terminal helicalsearch EF-hand domain pairsearch Zinc finger, RING/FYVE/PHD-typesearch Adaptor protein Cbl, EF hand-likesearch Adaptor protein Cbl, SH2-likesearch Zinc finger, RING-type, conserved sitesearch Adaptor protein Cbl, PTB domainsearch Adaptor protein Cblsearch
C Ubiquitin-conjugating enzyme E2search Ubiquitin-conjugating enzyme/RWD-likesearch Ubiquitin-conjugating enzyme, active sitesearch