› 4a12

X-ray diffraction
3.15Å resolution

Structure of the global transcription regulator FapR from Staphylococcus aureus in complex with DNA operator

Released:
DOI: 10.2210/pdb4a12/pdb
PDB entry 4a12 coloured by chain, front view.
PDB entry 4a12 coloured by chain, side view.
PDB entry 4a12 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for feed-forward transcriptional regulation of membrane lipid homeostasis in Staphylococcus aureus.
Albanesi D, Reh G, Guerin ME, Schaeffer F, Debarbouille M, Buschiazzo A, Schujman GE, de Mendoza D, Alzari PM
PLoS Pathog 9 e1003108 (2013)
PMID: 23300457

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117185 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor FapR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 22.12 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q2FZ56 (Residues: 1-190; Coverage: 100%)
Gene names: SAOUHSC_01196, fapR
Sequence domains: Thioesterase superfamily
Structure domains:
FAPR PROMOTER Chain: F
Molecule details ›
Chain: F
Length: 40 nucleotides
Theoretical weight: 12.2 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
FAPR PROMOTER Chain: G
Molecule details ›
Chain: G
Length: 40 nucleotides
Theoretical weight: 12.42 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 114.69Å b: 249.94Å c: 179.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.201
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

9 review citations

Bacterial lipids: metabolism and membrane homeostasis.
Parsons et al. (2013)
8 more

14 mentions without citation

Potent Molecular Feature-based Neutralizing Monoclonal Antibodies as Promising Therapeutics Against SARS-CoV-2 Infection.
Kombe Kombe et al. (2021)
13 more