› 4a10

X-ray diffraction
2.25Å resolution

Apo-structure of 2-octenoyl-CoA carboxylase reductase CinF from streptomyces sp.

Released:
DOI: 10.2210/pdb4a10/pdb
PDB entry 4a10 coloured by chain, front view.
PDB entry 4a10 coloured by chain, side view.
PDB entry 4a10 coloured by chain, top view.
Source organism: Streptomyces cinnabarigriseus
Primary publication:
Unusual carbon fixation gives rise to diverse polyketide extender units.
Quade N, Huo L, Rachid S, Heinz DW, Müller R
Nat Chem Biol 8 117-24 (2011)
PMID: 22138621

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl reductase (ER) domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 447 amino acids
Theoretical weight: 48.03 KDa
Source organism: Streptomyces cinnabarigriseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F0V3Z3 (Residues: 2-448; Coverage: 100%)
Gene name: cinF
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 101.64Å b: 85.22Å c: 113.97Å
α: 90° β: 107.45° γ: 90°
R-values:
R R work R free
0.212 0.209 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Decoding and reprogramming complex polyketide assembly lines: prospects for synthetic biology.
Hertweck C. (2015)
7 more

25 mentions without citation

Modulation of Signaling Mediated by TSLP and IL-7 in Inflammation, Autoimmune Diseases, and Cancer.
Marković et al. (2020)
24 more