› 4zsx

X-ray diffraction
2.19Å resolution

Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-2.0

Released:
DOI: 10.2210/pdb4zsx/pdb
PDB entry 4zsx coloured by chain, front view.
PDB entry 4zsx coloured by chain, side view.
PDB entry 4zsx coloured by chain, top view.
Source organisms:
Primary publication:
On the predictability of the orientation of protein domains joined by a spanning alpha-helical linker.
Lai YT, Jiang L, Chen W, Yeates TO
Protein Eng Des Sel 28 491-9 (2015)
PMID: 26243886

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF1122 domain-containing protein; Four-helix bundle copper-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 34.2 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67778 (Residues: 1-196; Coverage: 98%)
  • Canonical: Q9I208 (Residues: 34-130; Coverage: 74%)
Gene names: PA2107, aq_1966
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R3
Unit cell:
a: 121.43Å b: 121.43Å c: 207.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.228 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Geometric Principles for Designing Highly Symmetric Self-Assembling Protein Nanomaterials.
Yeates TO. (2017)
5 more