› 4zkn

X-ray diffraction
1.36Å resolution

The crystal structure of upain-1-W3A in complex with uPA at pH5.5

Released:
DOI: 10.2210/pdb4zkn/pdb
PDB entry 4zkn coloured by chain, front view.
PDB entry 4zkn coloured by chain, side view.
PDB entry 4zkn coloured by chain, top view.
Source organisms:
Entry authors: Jiang L, Andreasen PA, Huang M

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133283 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 247 amino acids
Theoretical weight: 27.84 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00749 (Residues: 179-425; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
upain-1-W3A Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.38 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R3
Unit cell:
a: 121.049Å b: 121.049Å c: 42.979Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.228 0.265
Expression systems:
  • Komagataella pastoris
  • Not provided

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