› 4zb5

X-ray diffraction
2Å resolution

A form of glucose isomerase collected at 100K.

Released:
DOI: 10.2210/pdb4zb5/pdb
PDB entry 4zb5 coloured by chain, front view.
PDB entry 4zb5 coloured by chain, side view.
PDB entry 4zb5 coloured by chain, top view.
Source organism: Streptomyces rubiginosus
Primary publication:
A generic protocol for protein crystal dehydration using the HC1b humidity controller.
Lobley CM, Sandy J, Sanchez-Weatherby J, Mazzorana M, Krojer T, Nowak RP, Sorensen TL
Acta Crystallogr D Struct Biol 72 629-40 (2016)
PMID: 27139626

Function and Biology Details

Reaction catalysed: 
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.28 KDa
Source organism: Streptomyces rubiginosus
Expression system: Streptomyces rubiginosus
UniProt:
  • Canonical: P24300 (Residues: 1-387; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand GLC 1 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: I222
Unit cell:
a: 92.904Å b: 98.152Å c: 102.704Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 0.115 0.154
Expression system: Streptomyces rubiginosus

Quick links

Citations

4 citation in other articles

Nylon mesh-based sample holder for fixed-target serial femtosecond crystallography.
Lee et al. (2019)
3 more

2 mentions without citation

Merging of synchrotron serial crystallographic data by a genetic algorithm.
Zander et al. (2016)
1 more