› 4z4z

X-ray diffraction
1.8Å resolution

Crystal structure of GII.10 P domain in complex with 30mM B antigen (trisaccharide)

Released:
DOI: 10.2210/pdb4z4z/pdb
PDB entry 4z4z coloured by chain, front view.
PDB entry 4z4z coloured by chain, side view.
PDB entry 4z4z coloured by chain, top view.
Source organism: Norwalk virus
Primary publication:
The sweet quartet: Binding of fucose to the norovirus capsid.
Koromyslova AD, Leuthold MM, Bowler MW, Hansman GS
Virology 483 203-8 (2015)
PMID: 25980740

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Capsid protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 34.51 KDa
Source organism: Norwalk virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5F4T5 (Residues: 224-538; Coverage: 58%)
Sequence domains: Calicivirus coat protein C-terminal
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLA, FUC
Carbohydrate polymer
1 bound ligand:
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P21
Unit cell:
a: 66.83Å b: 78.293Å c: 71.63Å
α: 90° β: 103.35° γ: 90°
R-values:
R R work R free
0.165 0.164 0.193
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Human Norovirus Interactions with Histo-Blood Group Antigens and Human Milk Oligosaccharides.
Schroten et al. (2016)
4 more

1 mention without citation

Nanobodies targeting norovirus capsid reveal functional epitopes and potential mechanisms of neutralization.
Koromyslova et al. (2017)