› 4z1w

X-ray diffraction
1.3Å resolution

CRYSTAL STRUCTURE OF MONOMERIC BACTERIOPHYTOCHROME mutant D207L Y263F From Synchrotron

Released:
DOI: 10.2210/pdb4z1w/pdb
PDB entry 4z1w coloured by chain, front view.
PDB entry 4z1w coloured by chain, side view.
PDB entry 4z1w coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
Removal of Chromophore-Proximal Polar Atoms Decreases Water Content and Increases Fluorescence in a Near Infrared Phytofluor.
Lehtivuori H, Bhattacharya S, Angenent-Mari NM, Satyshur KA, Forest KT
Front Mol Biosci 2 65 (2015)
PMID: 26636092

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 37.18 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RZA4 (Residues: 1-321; Coverage: 43%)
Gene names: DR_A0050, bphP
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LBV 1 x LBV
Ligand LBW 1 x LBW
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 94.434Å b: 53.34Å c: 65.69Å
α: 90° β: 90.91° γ: 90°
R-values:
R R work R free
0.145 0.145 0.16
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes.
Oliinyk et al. (2017)
10 other articles

1 mention without citation

Removal of Chromophore-Proximal Polar Atoms Decreases Water Content and Increases Fluorescence in a Near Infrared Phytofluor.
Lehtivuori et al. (2015)