› 4yue

X-ray diffraction
2.19Å resolution

Mouse IL-2 Bound to S4B6 Fab Fragment

Released:
DOI: 10.2210/pdb4yue/pdb
PDB entry 4yue coloured by chain, front view.
PDB entry 4yue coloured by chain, side view.
PDB entry 4yue coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Antibodies to Interleukin-2 Elicit Selective T Cell Subset Potentiation through Distinct Conformational Mechanisms.
Spangler JB, Tomala J, Luca VC, Jude KM, Dong S, Ring AM, Votavova P, Pepper M, Kovar M, Garcia KC
Immunity 42 815-25 (2015)
PMID: 25992858

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229831 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
S4B6 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 238 amino acids
Theoretical weight: 25.79 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
S4B6 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 227 amino acids
Theoretical weight: 24.73 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
Interleukin-2 Chain: C
Molecule details ›
Chain: C
Length: 130 amino acids
Theoretical weight: 14.96 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04351 (Residues: 47-169; Coverage: 83%)
Gene names: Il-2, Il2
Sequence domains: Interleukin 2
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 43.08Å b: 77.56Å c: 169.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.231
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

42 review citations

Treg cell-based therapies: challenges and perspectives.
Raffin et al. (2020)
41 more

5 mentions without citation

Antibodies to Interleukin-2 Elicit Selective T Cell Subset Potentiation through Distinct Conformational Mechanisms.
Spangler et al. (2015)
4 more