› 4ymk

X-ray diffraction
2.6Å resolution

Crystal Structure of Stearoyl-Coenzyme A Desaturase 1

Released:
DOI: 10.2210/pdb4ymk/pdb
PDB entry 4ymk coloured by chain, front view.
PDB entry 4ymk coloured by chain, side view.
PDB entry 4ymk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
X-ray structure of a mammalian stearoyl-CoA desaturase.
Bai Y, McCoy JG, Levin EJ, Sobrado P, Rajashankar KR, Fox BG, Zhou M
Nature 524 252-6 (2015)
PMID: 26098370

Function and Biology Details

Reaction catalysed: 
Stearoyl-CoA + 2 ferrocytochrome b5 + O(2) + 2 H(+) = oleoyl-CoA + 2 ferricytochrome b5 + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146632 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-CoA desaturase 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 339 amino acids
Theoretical weight: 39.57 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P13516 (Residues: 24-355; Coverage: 94%)
Gene name: Scd1
Sequence domains: Fatty acid desaturase

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand ST9 2 x ST9
Ligand MPG 2 x MPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 77.061Å b: 113.766Å c: 141.698Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.235
Expression system: Trichoplusia ni

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Citations

22 review citations

Lipid metabolism reprogramming and its potential targets in cancer.
Cheng et al. (2018)
21 more

25 mentions without citation

Dioxygen Activation by Nonheme Diiron Enzymes: Diverse Dioxygen Adducts, High-Valent Intermediates, and Related Model Complexes.
Jasniewski et al. (2018)
24 more