› 4ybo

X-ray diffraction
2.18Å resolution

Structure of Citrate Synthase from the Thermoacidophilic Euryarchaeon Thermolasma acidophilum

Released:
DOI: 10.2210/pdb4ybo/pdb
PDB entry 4ybo coloured by chain, front view.
PDB entry 4ybo coloured by chain, side view.
PDB entry 4ybo coloured by chain, top view.
Source organism: Thermoplasma acidophilum DSM 1728
Primary publication:
An active site-tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase.
Murphy JR, Donini S, Kappock TJ
Acta Crystallogr F Struct Biol Commun 71 1292-9 (2015)
PMID: 26457521

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 399 amino acids
Theoretical weight: 44.74 KDa
Source organism: Thermoplasma acidophilum DSM 1728
Expression system: Escherichia coli
UniProt:
  • Canonical: P21553 (Residues: 2-385; Coverage: 100%)
Gene names: Ta0169, gltA
Sequence domains: Citrate synthase, C-terminal domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BCT 2 x BCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 56.505Å b: 113.406Å c: 120.066Å
α: 90° β: 95.08° γ: 90°
R-values:
R R work R free
0.166 0.164 0.212
Expression system: Escherichia coli

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Citations

5 citation in other articles

A primordial and reversible TCA cycle in a facultatively chemolithoautotrophic thermophile.
Nunoura et al. (2018)
4 more

1 mention without citation

An active site-tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase.
Murphy et al. (2015)