› 4y99

X-ray diffraction
2Å resolution

Core domain of human cardiac troponin

Released:
DOI: 10.2210/pdb4y99/pdb
PDB entry 4y99 coloured by chain, front view.
PDB entry 4y99 coloured by chain, side view.
PDB entry 4y99 coloured by chain, top view.
Source organism: Homo sapiens
Entry author: Takeda S

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148573 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Troponin C, slow skeletal and cardiac muscles Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 18.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63316 (Residues: 1-161; Coverage: 100%)
Gene names: TNNC, TNNC1
Sequence domains:
Troponin T, cardiac muscle Chain: B
Molecule details ›
Chain: B
Length: 106 amino acids
Theoretical weight: 12.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45379 (Residues: 193-298; Coverage: 36%)
Gene name: TNNT2
Sequence domains: Troponin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Troponin I, cardiac muscle Chain: C
Molecule details ›
Chain: C
Length: 210 amino acids
Theoretical weight: 23.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19429 (Residues: 1-210; Coverage: 100%)
Gene names: TNNC1, TNNI3
Sequence domains:
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand DMS 6 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 66.062Å b: 80.368Å c: 93.712Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.259
Expression system: Escherichia coli

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