› 4xxj

X-ray diffraction
1.9Å resolution

Crystal Structure of Escherichia coli-Expressed Halobacterium salinarum Bacteriorhodopsin in the Trimeric Form

Released:
DOI: 10.2210/pdb4xxj/pdb
PDB entry 4xxj coloured by chain, front view.
PDB entry 4xxj coloured by chain, side view.
PDB entry 4xxj coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin.
Bratanov D, Balandin T, Round E, Shevchenko V, Gushchin I, Polovinkin V, Borshchevskiy V, Gordeliy V
PLoS One 10 e0128390 (2015)
PMID: 26046789

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 269 amino acids
Theoretical weight: 29.4 KDa
Source organism: Halobacterium salinarum
Expression system: Escherichia coli
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

2 bound ligands:
Ligand LFA 33 x LFA
Ligand MPG 3 x MPG
1 modified residue:
Ligand LYR 3 x LYR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 105.84Å b: 60.76Å c: 113.36Å
α: 90° β: 99.78° γ: 90°
R-values:
R R work R free
0.185 0.183 0.214
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.
de Grip et al. (2022)
1 more

2 mentions without citation

High-throughput stability screening for detergent-solubilized membrane proteins.
Kotov et al. (2019)
1 more